How to Read a Syringe

A small U-100 syringe is marked in units. Knowing what a unit is, and where to read the line, makes every other number on the site easier to check.

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Quick Answer

On a U-100 syringe, 100 units equal 1 mL, so 1 unit is 0.01 mL. The reading is the line level with the edge of the plunger tip that faces the needle.

25 units = 0.25 mL 0102030405060708090100 needle end: 0 units plunger end: 100 units
An example scale. The plunger tip sits at 25 units, which is 0.25 mL. Marks differ between brands.

What a Unit Is

A unit is a measure of volume, not of peptide. U-100 means 100 units fill 1 mL. The scale measures liquid only. How much peptide that liquid holds depends on the strength of the mix, which is why the mixing guide and the calculator go together with this page.

Syringe SizeTotal VolumeTotal UnitsOne Unit Is
0.3 mL0.3 mL30 units0.01 mL
0.5 mL0.5 mL50 units0.01 mL
1 mL1 mL100 units0.01 mL

Reading the Line

Hold It Level

Researchers commonly read a syringe at eye level, with the barrel horizontal or upright. Common practice

Find the Plunger Tip

The tip is the dark rubber part inside the barrel. Use the edge that faces the needle.

Count What Each Mark Is Worth

Marks differ between brands. Check how many units sit between two numbered lines, then count from the nearest number.

Convert to Millilitres

Divide the units by 100. A reading of 25 units is 0.25 mL.

Units and Millilitres

UnitsMillilitres
50.05 mL
100.10 mL
250.25 mL
500.50 mL
1001.00 mL
Worked example At 5 mg/mL, 0.05 mL (5 units) holds 0.25 mg, which is 250 mcg. At 2.5 mg/mL the same 5 units holds 0.125 mg, or 125 mcg. The syringe reading is the same, and the amount of peptide is different. The strength decides the amount.
For Research Use Only This page reports what published studies and researchers have found. It is information, not advice.